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Merck

B4679

Sigma-Aldrich

BIS-TRIS-Propan

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(e):

1,3-Bis[tris(hydroxymethyl)methylamino]propan

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About This Item

Lineare Formel:
CH2[CH2NHC(CH2OH)3]2
CAS-Nummer:
Molekulargewicht:
282.33
Beilstein:
1786109
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12161700
PubChem Substanz-ID:
NACRES:
NA.25

Qualität

BioPerformance Certified

Qualitätsniveau

Assay

≥99.0%

Form

crystals

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin and bioburden, tested

Nützlicher pH-Bereich

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp (Schmelzpunkt)

164-165 °C (lit.)

Anwendung(en)

diagnostic assay manufacturing

Fremdaktivität

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES String

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChIKey

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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Anwendung

  • Neutralized chimeric DNA probe for the improvement of GC-rich RNA detection specificity on the nanowire field-effect transistor.: BIS-TRIS propane is used in the development of nanowire field-effect transistors for detecting GC-rich RNA sequences. The study demonstrates the buffer′s role in enhancing the specificity and sensitivity of these biosensors (Chou et al., 2019).

  • Elimination of ghost peaks by optimization of anion exchange chromatography method for determination of gamma-carboxyglutamic acid (Gla)-domainless impurity in recombinant activated clotting factor VII drug products.: BIS-TRIS propane is employed in optimizing chromatography methods to eliminate ghost peaks during the analysis of pharmaceutical impurities. The buffer′s effectiveness ensures accurate and reliable detection of impurities in drug formulations (Tahmasbi et al., 2019).

Sonstige Hinweise

Easily compare specifications for BIS-TRIS propane products with the BIS-TRIS propane specification table.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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R Coronado et al.
The Journal of general physiology, 79(4), 529-547 (1982-04-01)
A collection of organic cations has been used to probe the gross structural features of the ionic diffusion pathway in a K+-selective channel from sarcoplasmic reticulum (SR). Channels were incorporated into planar phospholipid bilayer membranes, and single-channel currents were measured
R Tóth-Boconádi et al.
Biophysical journal, 78(6), 3170-3177 (2000-05-29)
Buffers change the electric signals of light-excited bacteriorhodopsin molecules in purple membrane if their concentration and the pH of the low-salt solution are properly selected. "Positive" buffers produce a positive component, and "negative" buffers a negative component in addition to
Michael C Breadmore
Journal of chromatography. A, 1217(24), 3900-3906 (2010-05-11)
An EOF counter-balanced ITP boundary has been used to stack anions from high conductivity samples during continuous electrokinetic injection of the sample. In a polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, the time at which the ITP boundary exited the capillary could
S J Smith et al.
The Journal of biological chemistry, 259(15), 9365-9368 (1984-08-10)
The kinetics of binding and hydrolysis of ATP by bovine cardiac myosin subfragment 1 has been reinvestigated. More than 90% of the total fluorescence amplitude associated with ATP hydrolysis occurs with an apparent second-order rate constant of 8.1 X 10(5)
Sami J O Varjo et al.
Journal of chromatography. A, 1081(1), 92-98 (2005-07-15)
Greater stability of liposome coatings and improved resolution of model steroids in capillary electrochromatography (CEC) were sought by adding small diamines (ethylenediamine, diaminopropane, bis-tris-propane, or N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid, HEPES)) to the liposome solution before coating of fused silica capillaries. The phospholipid

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