APOAC

Annexin V-Cy3^™ Apoptosis Detection Kit

• Synonym(e): Red fluorescent Annexin V apoptosis assay
• UNSPSC-Code: 12352207
• NACRES: NA.32

Eigenschaften

• Verwendung: sufficient for 200 tests
• Qualitätsniveau: 200
• Verpackung: pkg of 1 kit
• Methode(n): flow cytometry: suitable
• Anwendung(en): cell analysis; detection
• Nachweisverfahren: fluorometric
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Leistungsmerkmale und Vorteile

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL

• Rapid labeling of cells. Cell staining takes only 10 minutes.
• No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
• 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Prinzip

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Rechtliche Hinweise

Cy3 is a trademark of Cytiva

Nur Kit-Komponenten

• Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Kit-Komponenten auch einzeln erhältlich

• C5041 6-Carboxyfluorescein diacetate 10 mg

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable