As per the Product Information Sheet, the Accutase solution does not contain mammalian or bacterial-derived products. This solution is supplied as a sterile, ready-to use, frozen liquid. This product is serum free.
Wichtige Dokumente
A6964
Accutase® -Lösung
sterile-filtered, suitable for cell culture
Synonym(e):
Cell detachment solution
About This Item
Empfohlene Produkte
Sterilität
sterile-filtered
Qualitätsniveau
Form
liquid
Konzentration
400-600 units/mL
Methode(n)
cell culture | mammalian: suitable
single cell analysis: suitable
Verunreinigungen
≤1 EU/mL endotoxin
pH-Wert
6.8-7.8
Versandbedingung
dry ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Anwendung
Geeignet zur Verwendung bei der Herstellung einer Einzelzellsuspension für die Sequenzierung.
Leistungsmerkmale und Vorteile
Alternative Formulierung
Angaben zur Herstellung
Rechtliche Hinweise
Ähnliches Produkt
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Is Accutase Solution A6964 animal-origin free? Is serum free?
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Can accutase be thawed, aliquoted, and refrozen at -20 to preserve it longer?
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Yes, this product is stable after undergoing several freeze/thaw cycles. The thawed product may be aliquoted and stored at –20 °C or stored for up to 2 months at 4°C.
The graphic below demonstrates the freeze/thaw stability of Accutase.Helpful?
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On the Innovative cell technologies website it says that accumax is the same as accutase but with 3x the amount of enzymes. Why did the technician here answer that it contains DNAse?
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Thank you for calling this to our attention. The manufacturer, Innovative Cell Technologies, has been contacted regarding this matter. The manufacturer has confirmed that both Accutase and Accumax products DO contain a small amounts of DNAse. The concentration is not measured, but it is present in the formulation. In addition, both products also contain 0.5 mM EDTA.
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How can I inactivate Accutase® with fetal bovine serum?
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Due to its gentle nature Accutase® cell detachment solution usually does not need to be neutralized following cell passaging. Accutase® is also heat inactivated after 45 minutes at 37°C. If this method of neutralization does not work for your cells, or if you want to neutralize Accutase® in a shorter period of time, the addition of 10% FBS containing media will accomplish this task. Experiments have shown that diluting a sample of Accutase® with 10% with fetal bovine serum achieved an environment similar to that found in cell culture. Measurements of the enzyme activity in this diluted sample showed decreased activity over time, falling below that of the required level for cell detachment (>175 units/mL). After about 6 minutes of serum interaction, the enzyme activity of Accutase® was well below this level, demonstrating effective inhibition by FBS.
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Will Product A6964, Accutase® solution, work on tissue?
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This product is not tested for tissue, but it should work on soft tissue.
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Does Product A6964, Accutase® solution, contain animal components?
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Accutase is purified from a marine invertebrate. There are no other animal or bacterial derived proteins present.
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What is the difference between Accutase and Accumax?
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Accutase is a mixture of non-mammalian proteases. Accumax is accutase that has been augmented with DNase to reduce clumping associated with DNA release from damaged cells.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?
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Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells. The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27
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What is the concentration of Product A6964, Accutase® solution?
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This product is typically at a concentration of 400-600 units/mL.
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