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A3352

Acyl-Coenzym A-Synthetase aus Pseudomonas sp.

Name: Acyl-Coenzym A-Synthetase aus <I>Pseudomonas</I> sp.
Brand: SIGMA

≥2 units/mg protein

Synonym(e):

Acid: CoA ligase (AMP-forming)

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Packungsgröße	SKU	Verfügbarkeit	Preis

Über diesen Artikel

CAS-Nummer:

9013-18-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-747-5

MDL number:

MFCD00130343

EG-Nummer:

6.2.1.3 (BRENDA, IUBMB)

Specific activity:

≥2 units/mg protein

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Eigenschaften

form

powder

Quality Segment

200

specific activity

≥2 units/mg protein

shipped in

dry ice

storage temp.

−20°C

Beschreibung

General description

Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily. It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif.

Application

Acyl-coenzyme A Synthetase may be used to study fatty acid metabolism and lipid metabolism. It has been used to study its interaction with fatty acid transport proteins, which has been found to be involved in the efficient cellular uptake of long-chain fatty acids in adipocytes [1].

Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:

as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)
in in vitro fatty acylation assays
in the synthesis of (¹⁴C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (³H)Palmitate-CoA

Biochem/physiol Actions

Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells [1].

Acyl-coenzyme A (CoA) synthetase (ACS) enzyme catalyzes a two-step thioesterification reaction involving the conversion of free fatty acids (FAs) to CoA esters. The substrate for ACS varies from two carbon to 26 carbon FAs. It is involved in the activation of FAs for lipid metabolism and enables FA to participate in various cellular metabolic pathways.

Other Notes

1 U bildet 1.0 μmol AMP und Oleoyl-Coenzym A aus ATP und Oleat bei pH 8.1 und 25 °C in Anwesenheit von Coenzym A.

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Dieser Artikel	A1765	C9658	O4878
Sigma-Aldrich A3352 Acyl-coenzyme A Synthetase from Pseudomonas sp.	Sigma-Aldrich A1765 S-Acetyl-coenzyme A synthetase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich C9658 Carboxypeptidase G from Pseudomonas sp.	Sigma-Aldrich O4878 Oxaloacetate Decarboxylase from Pseudomonas sp.
specific activity ≥2 units/mg protein	specific activity ≥3 units/mg protein	specific activity ≥3 units/mg protein	specific activity ≥100 units/mg solid
form powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in dry ice	shipped in -	shipped in -	shipped in -
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200