A2172

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse

clone 5C5, ascites fluid

• Synonym(e): Mouse Anti-Actin
• MDL-Nummer: MFCD00164521
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: ascites fluid
• Antikörper-Produkttyp: primary antibodies
• Klon: 5C5, monoclonal
• Enthält: 15 mM sodium azide
• Speziesreaktivität: rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog
• Methode(n): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle; immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle; indirect ELISA: suitable; western blot: suitable
• Isotyp: IgM
• UniProt-Hinterlegungsnummer: P68032; P68133
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ACTA1(58) , ACTC1(70) ; mouse ... Rwdd2a(69519) ; rat ... Acta1(29437) , Actc1(29275)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-α-Sarcomeric Actin (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins and the isoforms show >90% overall sequence homology. It is expressed in mammals and birds as six isoforms. Four of the six represent differentiation markers of muscle tissues. The other two are found in practically all cells.

Immunogen

rabbit striated muscle actin.

Anwendung

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has also been used

• immunofluorescence
• double immunostaining
• double labelling terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunofluorescence staining.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications.

Biochem./physiol. Wirkung

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Physikalische Form

The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.

Sonstige Hinweise

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable