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Merck

47140

Sigma-Aldrich

4-Fluor-7-nitrobenzofurazan

BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Synonym(e):

NBD-F

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About This Item

Empirische Formel (Hill-System):
C6H2FN3O3
CAS-Nummer:
Molekulargewicht:
183.10
Beilstein:
1077571
MDL-Nummer:
UNSPSC-Code:
12352100
PubChem Substanz-ID:
NACRES:
NA.32

Produktlinie

BioReagent

Qualitätsniveau

Assay

≥98.0% (HPLC)

mp (Schmelzpunkt)

52-54 °C (lit.)

Fluoreszenz

λex 470 nm; λem 528 nm in ethanol
λex 470 nm; λem 550 nm (Amino acid adducts)

Eignung

suitable for fluorescence

Lagertemp.

2-8°C

SMILES String

[O-][N+](=O)c1ccc(F)c2nonc12

InChI

1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

InChIKey

PGZIDERTDJHJFY-UHFFFAOYSA-N

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Anwendung

4-Fluoro-7-nitrobenzofurazan (NBD-F) is used as a protein, amino acid and drug fluorescent labeling reagent in HPLC pre-column derivatization procedures.
Fluoreszenzmarker für Aminosäuren und Amine in HPLC

Verpackung

Bottomless glass bottle. Contents are inside inserted fused cone.

Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Eye Irrit. 2 - Skin Irrit. 2

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

235.4 °F - closed cup

Flammpunkt (°C)

113 °C - closed cup

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves


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Kunden haben sich ebenfalls angesehen

Yang-Ki Park et al.
Journal of chromatography. A, 1217(20), 3357-3361 (2010-04-13)
Single drop microextraction (SDME) can be in-line coupled with capillary electrophoresis by attaching a drop to the tip of a capillary. With a 2-layer drop comprised of an aqueous basic acceptor phase covered with a thin organic layer, acidic analytes
Yanting Song et al.
Amino acids, 42(5), 1897-1902 (2011-04-21)
A high-performance liquid chromatography method in which fluorescence detection is used for the simultaneous determination of 21 amino acids is proposed. Amino acids were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and then separated on a monolithic silica column (MonoClad C18-HS, 150 mm×3
Nasr Y Khalil
Talanta, 80(3), 1251-1256 (2009-12-17)
A fully automated, rapid and highly sensitive HPLC method with automated sample pre-treatment by column-switching system and fluorescence detection has been developed for the trace quantitative determination of the new antidepressant reboxetine (RBX) in human plasma. A simple pre-column derivatization
Chiaki Aoyama et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 25(1), 63-65 (2009-01-14)
A simple and sensitive determination method for selenomethionine (Se-Met) using an HPLC-fluorescence detection system coupled with an on-line electrochemical reactor has been developed. NBD-F (4-fluoro-7-nitro-2,1,3-benzoxadiazole) was used as the fluorescent derivatization reagent for Se-Met. NBD-Se-Met was separated from NBD-derivatives of
Hua-Ming Tseng et al.
Electrophoresis, 30(8), 1399-1405 (2009-03-26)
A sensitive CE with LIF method has been developed for quantitative analysis of small carbohydrates. In this work, 17 carbohydrates including mono-, di- and oligosaccharides were simultaneously derivatized with 4-fluoro-7-nitrobenzofurazane (NBD-F) via a two-step reaction involving reductive amination with ammonia

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