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41969

Sigma-Aldrich

5-Carboxy-X-rhodamin-N-succinimidylester

BioReagent, suitable for fluorescence

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About This Item

Empirische Formel (Hill-System):
C37H33N3O7
CAS-Nummer:
209734-74-7
Molekulargewicht:
631.67
MDL-Nummer:
MFCD07437880
UNSPSC-Code:
12352116
PubChem Substanz-ID:
329756168
NACRES:
NA.32

Produktlinie

BioReagent

Qualitätsniveau

100

Form

powder

Löslichkeit

DMF: soluble
acetonitrile: soluble

Fluoreszenz

λex 575 nm; λem 600 nm in 0.1 M phosphate pH 7.0

Eignung

suitable for fluorescence

Lagertemp.

−20°C

SMILES String

[O-]C(=O)c1cc(ccc1-c2c3cc4CCCN5CCCc(c45)c3[o+]c6c7CCCN8CCCc(cc26)c78)C(=O)ON9C(=O)CCC9=O

InChI

1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(26(19-22)36(43)44)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2

InChIKey

BTTBJYLMDASSAS-UHFFFAOYSA-N

Anwendung

5-Carboxy-X-rhodamine N-succinimidyl ester (ROX-SE) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications.Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing.

Sonstige Hinweise

Labeling of oligonucleotides

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves

Analysenzertifikate (COA)

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Patrick J Finn et al.
Nucleic acids research, 30(13), 2877-2885 (2002-06-28)
A novel series of charge-modified, dye-labeled 2',3'-dideoxynucleoside-triphosphate terminators were synthesized and evaluated as reagents for DNA sequencing. These terminators possess an advantage over existing reagents in that no purification is required to remove unreacted nucleotide or associated breakdown products prior
Ting Qiu et al.
The Analyst, 135(9), 2394-2399 (2010-08-03)
A new strategy for quantitatively detecting micrococcal nuclease (MNase) is proposed using electrostatic interaction-based fluorescence resonance energy transfer (FRET) between positively charged QDs and negatively charged dye-labeled single-stranded DNA (dye-ssDNA). Herein, we have made our attempt to develop a strategy
Xia Xiang et al.
Analytica chimica acta, 751, 155-160 (2012-10-23)
The development of simple and inexpensive DNA detection strategy is very significant for droplet-based microfluidic system. Here, a droplet-based biosensor for multiplexed DNA analysis is developed with a common imaging device by using fluorescence-based colorimetric method and a graphene nanoprobe.
Yunfeng Xia et al.
The Analyst, 137(24), 5705-5709 (2012-10-27)
We report a sensitive approach for SERS detection of cytochrome c using target binding-induced conformational changes of signal transduction probe (STP). STP labeled with a SERS-active molecule, carboxy-X-rhodamine (ROX), is immobilized on the substrate where the formation of a rigid
Jonathan F Lovell et al.
Journal of the American Chemical Society, 133(46), 18580-18582 (2011-10-27)
Photodynamic therapy (PDT) stands to benefit from improved approaches to real-time treatment monitoring. One method is to use activatable photosensitizers that can both induce cell death (via singlet oxygen) and monitor it (via caspase detection). Here, we report porphyrins as
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