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Merck

T4375

Sigma-Aldrich

Tetrazoliumblauchlorid

used in colorimetric determination of reducing compounds

Synonym(e):

3,3′-(3,3′-Dimethoxy-[1,1′-biphenyl]-4,4′-diyl)-bis-(2,5-diphenyl-2H-tetrazolium)-dichlorid, 3,3′-Dianisol-4,4′-bis-(3,5-diphenyltetrazolium-chlorid), BTC, Blau Tetrazolium-(chlorid)

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About This Item

Empirische Formel (Hill-System):
C40H32Cl2N8O2
CAS-Nummer:
Molekulargewicht:
727.64
Beilstein:
3894077
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12171500
PubChem Substanz-ID:
NACRES:
NA.47

Qualitätsniveau

Form

powder

mp (Schmelzpunkt)

255 °C (dec.) (lit.)

Löslichkeit

methanol: 50 mg/mL, clear

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

room temp

SMILES String

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccccc3)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccccc7)-c8ccccc8

InChI

1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2

InChIKey

MUUHXGOJWVMBDY-UHFFFAOYSA-L

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Anwendung

Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Carc. 1B

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

Sensitive colorimetric assay of carbidopa and methyldopa using tetrazolium blue chloride in pharmaceutical products.
Issopoulos, P.B.
International Journal of Clinical Pharmacy, 11, 213-217 (1989)
Ternary complex of molybdenum(VI) with 4-nitrocatechol and tetrazolium blue chloride and Its application to extraction-spectrophotometric analysis of ferrous metallurgy products.
Dimitrov., A.N., et al.
Journal of Analytical Chemistry, 62, 122-125 (2007)
[Development of a novel Francisella tularensis antigen stained with tetrazolium-blue for tularemia microagglutination test].
Celebi B
Mikrobiyoloji bulteni (2013)
C K Jue et al.
Journal of biochemical and biophysical methods, 11(2-3), 109-115 (1985-08-01)
Addition of sodium potassium tartrate to basic solutions of tetrazolium blue [(2,2',5,5'-tetraphenyl-3,3'-dimethoxy 4,4'-biphenylene) ditetrazolium chloride] greatly improved their efficacy as colorimetric reagents for reducing sugar determination. This modification increased sensitivity and decreased reaction time. The modified reagent can determine as
Preparation of a new tetrazolium salt which yields a blue pigment on reduction and its use in the demonstration of enzymes in normal and neoplastic tissues.
A M RUTENBURG et al.
Cancer research, 10(2), 113-121 (1950-02-01)

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