PPB002

Tris Buffered Saline

powder, pH 8.0, pHast Pack^™, with 0.05% Tween^® 20

• Synonym(e): Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST
• UNSPSC-Code: 41105323
• NACRES: NA.21

Eigenschaften

• Produktbezeichnung: Trisgepufferte Kochsalzlösung, mit Tween™ 20, pH 8,0, powder, pHast Pack^™
• Produktlinie: pHast Pack^™
• Form: powder
• Lagerbedingungen: (Tightly closed. Dry.)
• Verunreinigungen: ≤5 ppm Heavy Metals (lead)
• Farbe: white
• pH-Wert: 7.8—8.2
• Löslichkeit: soluble, clear to hazy, colorless
• Kationenspuren: Fe: ≤10 ppm
• Fremdaktivität: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Tris Buffered Saline with 0.05% Tween^® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.

Anwendung

Suitable for use as a wash buffer in:

• Western blot
• ELISA
• Immunohistochemistry

Leistungsmerkmale und Vorteile

• Ready-to-use buffer requires no measurments or pH adjustments
• Saves time, money and effort to get your assays started fast
• Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
• Qualified for use in gel electrophoreis and other molecular biology assays

Verpackung

Foil pouches

Rekonstituierung

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Lagerung und Haltbarkeit

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Sonstige Hinweise

For additional information on our range of Biochemicals, please complete this form.

Rechtliche Hinweise

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable