B0149

Brillantblau R

Dye content ~50 %, Powder

• Synonym(e): Brillantindocyanin 6B, Coomassie Brillantblau R 250, Kensäure Blau R
• Empirische Formel (Hill-System): C₄₅H₄₄N₃NaO₇S₂
• CAS-Nummer: 6104-59-2
• Molekulargewicht: 825.97
• Farbindexnummer: 42660
• Beilstein: 5718025
• EG-Nummer: 228-060-5
• MDL-Nummer: MFCD00041762
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24891505
• NACRES: NA.47

Eigenschaften

• product name: Brillantblau R, Dye content ~50 %, Technical grade
• Qualitätsniveau: 200
• Form: powder
• Zusammensetzung: Dye content, ~50%
• Methode(n): microbe id | staining: suitable
• Löslichkeit: H₂O: 10 mg/mL
• Eignung: suitable for detection of protein bands following electrophoresis.
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• SMILES String: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• InChI: 1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
• InChIKey: NKLPQNGYXWVELD-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250.

Anwendung

Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).Its other applications include:

• as a protein stain on epoxy-embedded tissue sections
• for quantitative micro densitometry of protein in sections
• for staining chromatids, actinin microfilaments, developing neurites, and sperm acrosomes.

Nachweis der Proteinbanden nach Elektrophorese

Rechtliche Hinweise

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)