70191
Mueller-Hinton-Agar
suitable for microbiology, NutriSelect® Plus
Synonym(e):
MH agar; Antibiotic sensitivity test media; disc diffusion test media
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Alle Fotos(1)
About This Item
UNSPSC-Code:
41171607
NACRES:
NA.74
Empfohlene Produkte
Sterilität
non-sterile
Qualitätsniveau
Produktlinie
BioChemika
Form
powder
Haltbarkeit
limited shelf life, expiry date on the label
Zusammensetzung
agar, 17.0 g/L
beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L
Hersteller/Markenname
NutriSelect® Plus
Methode(n)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
pH-Endwert
7.3±0.2 (25 °C)
Anwendung(en)
clinical testing
food and beverages
microbiology
Lagertemp.
10-30°C
Eignung
nonselective for Neisseria spp.
nonselective for bacteria (General Media)
Allgemeine Beschreibung
Mueller Hinton Agar is specially designed for antibiotic susceptibility testing using the disc diffusion method (Kirby Bauer method). It has been recommended by the CLSI as the ideal medium for antibiotic susceptibility testing for the following reasons:
- It shows good reproducibility from batch to batch
- It is low in certain inhibitors like trimethoprim, sulfonamide and tetracycline
- It supports the growth of both fastidious and non-fastidious bacterial pathogens
- The starch in the media absorbs toxins thus preventing them from interfering with the antibiotics
- It is a loose agar and therefore facilitates better diffusion of antibiotics
- In culturing Escherichia coli ATCC™ 25922 strain for disc diffusion studies
- In disk diffusion assay of fusidic acid against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli
- In agar diffusion assay of drug-loaded nano-hydroxyapatite formulations against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli
Anwendung
Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens.
Angaben zur Herstellung
Suspend 38 g in 1 litre of distilled water, bring to the boil to dissolve the medium completely and sterilize by autoclaving at 121°C for 15 minutes.
Sonstige Hinweise
Enhancement of cefotoxamine and other cephalosporins against Enterococcus faecalis by blood supplemented Mueller-Hinton agar
Fußnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Rechtliche Hinweise
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Journal of drug delivery science and technology, 52, 110-121 (2019)
R D Jenkins et al.
Journal of clinical microbiology, 22(3), 369-374 (1985-09-01)
Disk diffusion susceptibility tests for enterococci are frequently modified by adding 5% sheep blood (SB) to Mueller-Hinton agar; the performance standards from the National Committee for Clinical Laboratory Standards sanction this addition. Susceptibility testing of aminoglycoside antibiotics is not recommended
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Placente D, et al.
International Journal of Pharmaceutics, 548(1), 559-570 (2018)
G M Eliopoulos et al.
Diagnostic microbiology and infectious disease, 12(2), 149-156 (1989-03-01)
The activities of cefotaxime and other aminothiazoyl oxime cephalosporins against Enterococcus faecalis were enhanced by addition of 5% sheep blood to Mueller-Hinton agar. This effect was not seen with aztreonam (aminothiazoyl oxime monobactam), cefotiam (aminothiazoyl, nonoxime), or other cephalosporins, and
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