199664

Sudanschwarz B

certified by the Biological Stain Commission

• Synonym(e): Ceresschwarz BN, Fettschwarz BN, Neptunschwarz, Solvent Black 3
• Empirische Formel (Hill-System): C₂₉H₂₄N₆
• CAS-Nummer: 4197-25-5
• Molekulargewicht: 456.54
• Farbindexnummer: 26150
• Beilstein: 723248
• EG-Nummer: 224-087-1
• MDL-Nummer: MFCD00006919
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24851932
• NACRES: NA.47

Eigenschaften

• Qualität: certified by the Biological Stain Commission
• Qualitätsniveau: 200
• Form: powder
• Methode(n): microbe id | staining: suitable
• Farbe: dark brown to very dark brown
• mp (Schmelzpunkt): 120-124 °C (lit.)
• λmax: 415 nm (2nd); 598 nm
• ε (Extinktionskoeffizient): ≥20000 at 596-603 nm in ethanol
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• SMILES String: CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
• InChI: 1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
• InChIKey: YCUVUDODLRLVIC-VPHDGDOJSA-N

Beschreibung

Anwendung

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Biochem./physiol. Wirkung

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Eignung

Zertifiziert für die Fettfärbung nach der Churukian Variante des Lillie und Asburn-Verfahrens ("supersaturated isopropanol method"). Sudan III ersetzt Ölrot O in dem Verfahren. Burdon-Färbung für bakterielle Lipide.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Warning
• H-Sätze: H341
• P-Sätze: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Gefahreneinstufungen: Muta. 2
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)