11412272001
Roche
Transforming Growth Factor-β1, human (hTGF-β1)
recombinant (CHO cells)
Synonym(e):
hTGF
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352207
Empfohlene Produkte
Biologische Quelle
human
Qualitätsniveau
Rekombinant
expressed in CHO cells
Assay
>95% (SDS-PAGE)
Form
solution
Wirksamkeit
<0.1 ng/mL EC50
Mol-Gew.
25,000 Da
Verpackung
pkg of 1 mL (1 μg)
Hersteller/Markenname
Roche
Lagerbedingungen
avoid repeated freeze/thaw cycles
Verunreinigungen
<0.1 EU/μgtested (LAL test)
UniProt-Hinterlegungsnummer
Lagertemp.
−20°C
Angaben zum Gen
human ... TGFB1(7040)
Allgemeine Beschreibung
Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.
Spezifität
Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.
Anwendung
Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.
Biochem./physiol. Wirkung
Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.
Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.
Qualität
Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)
Sequenz
Chain Length 2 x 112 AA
Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.
Einheitendefinition
EC50 definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.
Angaben zur Herstellung
Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
nwg
Flammpunkt (°F)
does not flash
Flammpunkt (°C)
does not flash
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
α-MSH stimulation contributes to TGF-β1 production via MC1R-MITF signaling pathway in melanoma cell
Hayashi E, et al.
Inflammation and regeneration, 35(5), 244-254 (2015)
Periostin is essential for periodontal ligament remodeling during orthodontic treatment.
Xu HY, et al.
Molecular Medicine Reports, 15(4), 1800-1806 (2017)
Cloning, expression, and characterization of chicken transforming growth factor ?4
Pan, Hongjie, and Jaroslava Halper
Biochemical and Biophysical Research Communications, 24-30 (2003)
Barbara Chiavarina et al.
Theranostics, 11(4), 1626-1640 (2021-01-08)
Colorectal cancer (CRC) cells are traditionally considered unresponsive to TGFβ due to mutations in the receptors and/or downstream signaling molecules. TGFβ influences CRC cells only indirectly via stromal cells, such as cancer-associated fibroblasts. However, CRC cell ability to directly respond
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.