MAB6020

Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4

culture supernatant, clone 11B2-1A4, Chemicon^®

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: culture supernatant
• Antikörper-Produkttyp: primary antibodies
• Klon: 11B2-1A4, monoclonal
• Speziesreaktivität: rat
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P30099
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: rat ... Cyp11B1(500892)

Beschreibung

Spezifität

Rat p450 11beta-Hydroxylase

Immunogen

Aa 272-285 (KNVYRELAE GRQQS-C)

Anwendung

Research Category
Stoffwechsel

Research Sub Category
Enzyme & Biochemie

Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4 is an antibody against Cytochrome P450 11β-Hydroxylase for use in IH(P) & WB.

Western blot: 1:300

Immunohistochemistry (S.T.F. on paraffin embedded tissue required, traditional formalin fixation or paraformaldehyde fixation is not recommended. Microwave or pressure cooker antigen retrieval; blocking with a solution of 0.5% SDS, 2% milk and 5% NGS in Tris-HCL pH 7.6. Primary antibody is diluted in PBS or TBS typically with no SDS or NGS: 1:50-200, usually overnight refrigerated. After washing 4X with Tris buffer pH 7.6, secondary goat anti-mouse secondary (AP124B) is incubated (1:500-1:2000) diluted in a solution of 2% milk, 5% NGS, Tris-HCL pH 7.6 and 0.05% tween 20. Detection is via streptavidin HRP/DAB. Recommended counter stain is Gill′s Haematoxylin.

Optimal working dilutions must be determined by end user.

STF fixative is available from streck laboratories, www.streck.com).

Physikalische Form

Supernatant. Liquid in 0.1% sodium azide.

Lagerung und Haltbarkeit

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable