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Key Documents

MAB1340

Sigma-Aldrich

Anti-Collagen Type I Antibody, clone C11

ascites fluid, clone C11, Chemicon®

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

C11, monoclonal

Speziesreaktivität

human

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
immunohistochemistry: suitable

Isotyp

IgG

NCBI-Hinterlegungsnummer

NM_000088.3

UniProt-Hinterlegungsnummer

P02452

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... COL1A1(1277)

Spezifität

Recognizes both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and II in ELISA, RIA or immunoblotting.

Anwendung

Detect Collagen Type I using this Anti-Collagen Type I Antibody, clone C11 validated for use in ELISA, IH.
Immunohistochemical staining of 10 μm thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is not recommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.

ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.

Optimal working dilutions must be determined by the end user.

Physikalische Form

Immunoglobulin Purified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose. . Lyophilized, reconstitute with 1 mL of distilled water.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence.
Zoumi, A; Yeh, A; Tromberg, BJ
Proceedings of the National Academy of Sciences of the USA null
Jose M Gonzalez et al.
Scientific reports, 6, 21315-21315 (2016-02-18)
The contractile trabecular meshwork (TM) modulates aqueous humor outflow resistance and intraocular pressure. The primary goal was to visualize and quantify human TM contractile state by analyzing actin polymerization (F-actin) by 2-photon excitation fluorescence imaging (TPEF) in situ. A secondary
Patricia Khashayar et al.
3 Biotech, 7(5), 312-312 (2017-09-29)
This article explains a step-wise protocol to develop an electrochemical sensor to quantify serum levels of C-telopeptide (CTX) crosslinks also known as crosslaps in a matter of minutes and with high level of accuracy. The technique needs only one-step (incubation)
Human tendon stem cells better maintain their stemness in hypoxic culture conditions.
Zhang, J; Wang, JH
Testing null
Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.
Ding, Z; Huang, H
BMC Musculoskelet. Disord. null

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