MAB1072

Anti-Erythropoietin Antibody, clone 1C8

clone 1C8, Chemicon^®, from mouse

• Synonym(e): erythropoietin, Epoetin, epoetin
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Klon: 1C8, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• Isotyp: IgG2b
• NCBI-Hinterlegungsnummer: NM_000799.2
• UniProt-Hinterlegungsnummer: P01588
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.

Spezifität

Reacts with human Erythropoietin (Epo).

Immunogen

Recombinant human Epo.

Anwendung

Research Category
Stoffwechsel

Research Sub Category
Stoffwechselhormone & Rezeptoren

Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.

ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.

Immunosorbent preparation

Optimal working dilutions must be determined by the end user.

Qualität

Evaluated by Western Blot on Mouse kidney lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.

Zielbeschreibung

34 kDa (glycosylated)

Physikalische Form

Protein A purified

Format: Purified

Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8ºC from date of receipt.

Hinweis zur Analyse

Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable