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IP04

Millipore

Protein G Plus-Agarose Suspension

Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

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About This Item

UNSPSC-Code:
41116133
NACRES:
NA.56

Form

slurry (Liquid)

Enthält

≤0.1% sodium azide as preservative

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

do not freeze

Methode(n)

immunoprecipitation (IP): suitable

Eignung

suitable for immunoprecipitation

Versandbedingung

wet ice

Lagertemp.

2-8°C

Allgemeine Beschreibung

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for bovine, goat, human, and rat IgG and for mouse IgG1.
Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

Warnhinweis

Toxicity: Standard Handling (A)

Sonstige Hinweise

Agarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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