The diameter range of 4% beaded agarose typically falls between 50 to 165 micrometers.
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form
liquid
manufacturer/tradename
Upstate®
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable, western blot: suitable
shipped in
wet ice
General description
Application
Physical form
Preparation Note
Analysis Note
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| description Protein G Agarose, Fast Flow suitable for medium and low pressure chromatography of IgG from mouse, sheep, and rabbit, and for immunoprecipitations. | description Protein A Agarose Beads for the purification of human, mouse & rabbit immunoglobins. | description for use in chromatin immunoprecipitations (ChIP assays) | description >98% (HPLC and SDS-PAGE), suspension |
| technique(s) affinity chromatography: suitable, western blot: suitable, immunoprecipitation (IP): suitable | technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) ChIP: suitable | technique(s) - |
| form liquid | form liquid | form liquid | form suspension |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| manufacturer/tradename Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename Roche |
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Lagerklasse
3 - Flammable liquids
wgk
WGK 1
flash_point_f
116.6 °F
flash_point_c
47 °C
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What is the approximate diameter of each agarose bead?
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Is the 16-266 protein G column compatible with purifying IgG in the presence of FBS when purifying mAb, IgG2b, from cell culture supernatant containing FBS?
1 answer-
The presence of FBS in the cell culture supernatant containing IgG2b may lead to reduced yield and purity of the mAb, IgG2b, as FBS contains bovine immunoglobulins that can compete with the target antibody for binding to the protein G column.
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