HNCSMAG-35K
MILLIPLEX® Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay
About This Item
Empfohlene Produkte
Speziesreaktivität
human
Qualitätsniveau
Hersteller/Markenname
Milliplex®
assay range
accuracy: 129%
(Spike Recovery for Melatonin)
sensitivity: 4.3 pg/mL
(Melatonin; MinDC+2SD)
sensitivity: 686 pg/mL
(Cortisol; MinDC+2SD)
standard curve range: 3.4-2,500 pg/mL
(Melatonin)
standard curve range: 686-500,000 pg/mL
(Cortisol)
inter-assay cv: <25%
intra-assay cv: <15%
Methode(n)
multiplexing: suitable
Nachweisverfahren
fluorometric (Luminex xMAP)
Versandbedingung
wet ice
Allgemeine Beschreibung
The MILLIPLEX® Human Circadian/Stress Bead Panel is to be used for the simultaneous quantification of Cortisol and Melatonin. This is a configurable assay, and either or both analytes can be selected. This kit may be used for the analysis of human serum, plasma, tissue/cell lysate, culture supernatant samples, or cerebrospinal fluid (CSF). Serum or plasma samples should be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with this kit.
Panel Type: Neuroscience
Spezifität
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Anwendung
- Analytes: Cortisol, Melatonin
- Recommended Sample type: serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples
- NOTE: Sample extraction protocols are required for serum/plasma samples. Other sample types (e.g. CSF, culture supernatant, saliva, etc.) do not require sample extraction.
- Recommended Sample dilution: 1:4 for extracted serum and plasma or neat for CSF and culture supernatant
- Assay Run Time: Overnight
- Research Category: Neuroscience Metabolism
- Research Subcategory: Category Metabolic Disorders Obesity Inflammation & Autoimmune Mechanisms
- NOTE: This is a competitive assay
Leistungsmerkmale und Vorteile
Verpackung
Komponenten
Human Circadian/Stress Quality Control 1, 1 vial, lyophilized
Human Circadian/Stress Quality Control 2, 1 vial, lyophilized
Assay Buffer, 2 bottles, 30 mL ea
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Human Circadian/Stress Primary Antibodies, 1 bottle, 3.5 mL
Human Circadian/Stress Detection Antibodies, 1 bottle, 5.5 mL
Human Circadian/Stress Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
Lagerung und Haltbarkeit
Rechtliche Hinweise
Haftungsausschluss
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Lagerklassenschlüssel
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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