FCMAB133F

Milli-Mark^® Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate

clone TRA-2-49/6E, Milli-Mark^®, from mouse

• Synonym(e): Anti-TRA-2-49 FITC, Clone TRA-2-49 Antibody, TRA-2-49 Antibody
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: FITC conjugate
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: TRA-2-49/6E, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Milli-Mark^®
• Methode(n): flow cytometry: suitable
• Isotyp: IgG1
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Spezifität

Antibody recognizes TRA-2-49.

Immunogen

2102Ep human Embryonal Carcinoma Cells

Epitope: Unknown

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Pluripotente Stammzellen & Stammzellen der frühen Entwicklungsphase

Working Solution	Cells / test	Total Reaction Volume
5 μl	5 x 105 Cells / 95 μl PBS	100 μl
10 μl	1 x 106 Cells / 90 μl PBS	100 μl

Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.

Qualität

Evaluated by flow cytometry using 2102 Ep cells.

Zielbeschreibung

212/80/55 kDa Observed

Physikalische Form

Protein A purified

Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Lagerung und Haltbarkeit

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Hinweis zur Analyse

Control
2102 Ep cells.

Rechtliche Hinweise

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable