FCMAB103AP

Milli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate

clone Ki-S5, Milli-Mark^®, from mouse

• Synonym(e): MKI67 ; ; KIA ; ; Ki-67
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: Allophycocyanin conjugate
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: Ki-S5, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Milli-Mark^®
• Methode(n): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• Isotyp: IgG1
• NCBI-Hinterlegungsnummer: NM_002417.3
• UniProt-Hinterlegungsnummer: P46013
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MKI67(4288)

Beschreibung

Allgemeine Beschreibung

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin′s lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer.

Spezifität

Antibody recognizes Ki67.

Immunogen

Nuclear protein preparation from the human cell line U937.50

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur

Detect Ki67 using this Milli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate validated for use in FC, IC, IH, FC, IH(P).

Qualität

Evaluated by Flow Cytometry with Jurkat cells.

Zielbeschreibung

345 & 395 kDa observed

Physikalische Form

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Allophycocyanin (APC) in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Lagerung und Haltbarkeit

Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Hinweis zur Analyse

Control
Stimulated Jurkat Cells

Rechtliche Hinweise

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable