ABS403

Anti-phospho NFkB p65 Antibody, (Ser547)

from rabbit, purified by affinity chromatography

• Synonym(e): Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): chimpanzee (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_068810
• UniProt-Hinterlegungsnummer: Q04206
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer547)
• Angaben zum Gen: human ... RELA(5970)

Beschreibung

Allgemeine Beschreibung

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa B forms multiple homo and heterodimeric complexes, one of which is with RELA/p65. The NF-kappa-B p65 complex appears to be involved in invasin-mediated activation of IL-8 expression, as well as the inhibitor effect on I-kappa-B. Phosphorylation of p65 at serine 547 by the nuclear kinase ATM appears to repress NF-κB-dependent transcription of specific genes induced by genotoxic stress.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human phospho NFkB p65, (Ser547).

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Signalübertragung & Neurowissenschaft

Anti-phospho NFkB p65 Antibody, (Ser547) is an antibody against phospho NFkB p65 Antibody for use in Western Blotting.

Qualität

Evaluated by Western Blotting in p65 WT transfected U2OS cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho NFkB p65, (Ser547) in 10 µg of p65 WT transfected U2OS cell lysate.

Zielbeschreibung

~62 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable