AB9088

Anti-Polycystin-2 Antibody

serum, Chemicon^®

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: serum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NM_000297.2
• UniProt-Hinterlegungsnummer: Q13563
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PKD2(353503) ; mouse ... Pkd2(18764)

Beschreibung

Spezifität

Polycystin-2.

Immunogen

Synthetic peptide from the C-terminal of mouse Polycystin-2.

Anwendung

Research Category
Stoffwechsel

Research Sub Category
Nierenphysiologie

Anti-Polycystin-2 Antibody is an antibody against Polycystin-2 for use in WB, IC, IH.

Western blot: 1:500 using ECL. The antibody reacts with the 105 kDa Polycystin-2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

Physikalische Form

Rabbit serum. Liquid.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Control
POSITIVE CONTROL: adult mouse kidney

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable