AB5682

Anti-Neurogenin-2 Antibody

Chemicon^®, from rabbit

• Synonym(e): MATH4a (ms), ATH 4a, Atoh4, Ngn2, Atonal Protein Homolog 4
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: rat, mouse, human
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NM_024019.2
• UniProt-Hinterlegungsnummer: Q9H2A3
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NEUROG2(63973)

Beschreibung

Allgemeine Beschreibung

Neurogenin 2 (NGN2) is a helix-loop-helix class of transcription factor. Transcription factors with bHLH motifs modulate critical events in the development of the mammalian neocortex. The transition from proliferation to neurogenesis involves a coordinate increase in the activity of proneural bHLH factors, including Neurogenin 2 and a decrease in the activity of Hes and Id factors. bHLH factors have key roles in corticogenesis, affecting the timing of differentiation and the specification of cell fate. Neurog2 controls neurogenesis in the embryonic cerebral cortex directly induces the expression of the small GTP-binding protein Rnd2 in newly generated mouse cortical neurons before they initiate migration.

Spezifität

Recognizes Neurogenin 2 (MATH4a), a Helix-loop-helix class of transcription factor.

Immunogen

Synthetic peptide, amino acids 85-97 of mouse Neurogenin 2.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Entwicklungsneurowissenschaft

Neuronen- & Gliamarker

This Anti-Neurogenin-2 Antibody is validated for use in ELISA, IC, IH, IH(P), WB for the detection of Neurogenin-2.

Western blot: 1-10 μg/mL using ECL.
Immunocytochemistry: Cat. # AB5682 detected Neurogenin-2 in PC12 cells at 1:500 dilution. It was also detected in paraformaldehyde-fixed differentiated human neural stem cells.
ELISA: 0.5-1 μg/mL using 1 mg/mL control peptide (Catalog Number AG352) per well.
Immunohistochemistry on paraffin sections has been reported by Lamolet, B. et al. (2004)
Optimal working dilutions must be determined by the end user.

Zielbeschreibung

~28 kDa

Verlinkung

Replaces: AB9620

Physikalische Form

Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Lagerung und Haltbarkeit

Stable for 1 year at -70ºC from date of receipt.

Hinweis zur Analyse

Control
Human brain Lysate.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable