AB1632
Anti-Nitric Oxide Synthase I Antibody
Chemicon®, from rabbit
Synonym(e):
NOS I, bNOS, nNOS
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
affinity purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Aufgereinigt durch
affinity chromatography
Speziesreaktivität
bovine, mouse, rat
Hersteller/Markenname
Chemicon®
Methode(n)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
mouse ... Nos1(18125)
Spezifität
Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.
Immunogen
Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.
Anwendung
Research Category
Neurowissenschaft
Neurowissenschaft
Research Sub Category
Oxidativer Stress
Oxidativer Stress
Anti-Nitric Oxide Synthase I Antibody is an antibody against Nitric Oxide Synthase I for use in ELISA, WB, IH.
Immunohistochemistry: Use at 5 - 10 μg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons.
Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.
ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.
Optimal working dilutions must be determined by the end user.
Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.
ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.
Optimal working dilutions must be determined by the end user.
Physikalische Form
Peptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA.
Lagerung und Haltbarkeit
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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