654205-M

TNF-α, Mensch, rekombinant, E. coli

• Synonym(e): TNF-α, Mensch, rekombinant, E. coli, rhTNF-α, rh-Cachectin, Tumor-Nekrose-Faktor-α
• CAS-Nummer: 94948-59-1
• UNSPSC Code: 12352202
• NACRES: NA.27
• MDL number: MFCD00148692
• Form: lyophilized
• Assay: ≥95% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in E. coli

Eigenschaften

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• description: RTECS - MT6435000
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤0.1 ng/μg Endotoxin (ng/μg TNF-α)
• solubility: distilled water: 0.1—1 mg/mL
• storage temp.: −70°C
• Gene Information: human ... TNF(7124)

Beschreibung

General description

Research area: Apoptosis

Tumor Necrosis Factor alpha (TNF α), a homotrimer protein made of 157 amino acids, is produced by macrophages/monocytes, T-lymphocytes, and natural killer cells during acute inflammation. soluble and transmembrane form. The precursor form of TNF-α is in transmembrane form (tmTNF-α), which is processed to release soluble form (sTNF-α).

Application

TNF-α, Human, Recombinant, E. coli has been used to stimulate NF-κB activation and study the effects of pancreatic elastase on cellular responses.

Biochem/physiol Actions

Tumor Necrosis Factor alpha (TNF alpha), is an inflammatory cytokine. It coordinates a range of signaling events within cells, leading to necrosis or apoptosis. TNF-α initiates signal transduction pathways by binding to TNFR-1 and TNFR-2. These pathways cause cellular processes such as cell survival, differentiation, and proliferation.

Recombinant, human tumor necrosis factor-α expressed in E. coli., a cytokine produced primarily by activated macrophages, activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress-activated protein kinase (SAPK). ED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)

Preparation Note

Mit sterilem dH₂O bis zu einer Endkonzentration von 0,1–1 mg/ml rekonstituieren. Weitere Verdünnungen sollten in einem Medium mit niedrigem Endotoxingehalt oder in einem wässrigen Puffer vorgenommen werden, der ein Trägerprotein wie hitzeinaktivierte FCS oder BSA in Gewebekulturqualität enthält.

Nach der Rekonstitution in Aliquote aufteilen und für die langfristige Lagerung einfrieren (-20 °C oder -70 °C) oder für die kurzfristige Lagerung im Kühlschrank (4 °C) aufbewahren. Wiederholte Einfrier-Auftau-Zyklen der Lösungen vermeiden. Stammlösungen sind bis zu 3 Monate bei -20 °C oder -70 °C oder bis zu 1 Monat bei 4 °C stabil.

Other Notes

Merck Index 13, 9882.
Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994. Exp. Cell Res.214, 584.
Koide, M., et al. 1993. FEBS Lett.318, 213.
Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem.266, 7313.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxizität: Standard-Handhabung (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable