23-045-M

HIF-1α Protein, 10 µg

Recombinant human HIF-1α amino acids 331-609, N-terminal c-Myc, GST-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.

• Synonym(e): Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, bHLHe78, PAS domain-containing protein 8
• UNSPSC-Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.28

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in baculovirus infected Sf21 cells
• Hersteller/Markenname: Upstate^®
• Methode(n): activity assay: suitable
• NCBI-Hinterlegungsnummer: NM_001530

Beschreibung

Allgemeine Beschreibung

AXIS Axon Isolation Devices, 450 µm long channels, 10 devices

HIF-1α is the oxygen sensitive subunit of the hypoxia inducible factor HIF-1 which is an essential mediator of the cellular oxygen-signalling pathway. Under hypoxic conditions HIF-1α activates the transcription of over 40 genes, including those that control glucose uptake, metabolism, angiogenesis, erythropoiesis, cell proliferation, and apoptosis. The expression of HIF-1α is commonly increased in a variety of human tumours, and correlates with poor patient prognosis. Under normoxia this protein interacts with the ubiquitin ligase VHL complex, leading to its polyubiquitination and subsequent proteasomal degradation.

Anwendung

Ubiquitination Cascade Comoponent: Substrate

Recombinant human HIF-1α amino acids 331-609, N-terminal c-Myc, GST-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.

Physikalische Form

Purified using glutathione sepharose.

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317
• P-Sätze: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Gefahreneinstufungen: Skin Sens. 1
• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable