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Wichtige Dokumente
806595
APN-Azide
95%
Synonym(e):
3-(4-Azidophenyl)propiolonitrile, APN-N3
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About This Item
Empirische Formel (Hill-System):
C9H4N4
CAS-Nummer:
Molekulargewicht:
168.15
UNSPSC-Code:
12352125
PubChem Substanz-ID:
NACRES:
NA.22
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Qualitätsniveau
Assay
95%
Form
powder
Eignung der Reaktion
reagent type: cross-linking reagent
mp (Schmelzpunkt)
100 °C
Funktionelle Gruppe
azide
Lagertemp.
2-8°C
SMILES String
N#CC#CC1=CC=C(N=[N+]=[N-])C=C1
InChI
1S/C9H4N4/c10-7-1-2-8-3-5-9(6-4-8)12-13-11/h3-6H
InChIKey
BDLXITYEYYHQNK-UHFFFAOYSA-N
Anwendung
APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.
Standard protein labeling procedure (cysteine labeling)
1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
3. Incubate at room temperature for 2 hours.
4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.
5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.
*Note: avoid thiol-containing buffers.
Standard protein labeling procedure (cysteine labeling)
1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
3. Incubate at room temperature for 2 hours.
4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.
5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.
*Note: avoid thiol-containing buffers.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
4.1B - Flammable solid hazardous materials
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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