H1136 is not supplied in solution form. The product is lyophilized directly into the ampule and its appearance is a clear, thin film.
H1136
Hyaluronidase from Streptomyces hyalurolyticus
Synonym(s):
Hyaluronate Lyase from Streptomyces hyalurolyticus
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
biological source
Streptomyces sp. (S. hyalurolyticus)
Quality Level
conjugate
conjugate (Glucosaminoglycan)
form
film (Clear)
packaging
ampule of 300 × units
storage temp.
−20°C
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General description
Hyaluronidase is widely present in nature.[1] In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.[2]
Application
Hyaluronidase from Streptomyces hyalurolyticus has been used:
- in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue[2]
- as a component of digestion solution for the derivation of germline stem cells from testicular tissue[3]
Biochem/physiol Actions
Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.[4]
Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.[1]
Unit Definition
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Protocols
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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What is the volume in each vial/ampoule?
1 answer-
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You mention the following buffer composition for reconstitution: "...20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0". If I want to refreeze aliquots, would you suggest the same buffer? Isn't sodium phosphate harmful here?
1 answer-
Long-term solution stability studies have not been performed on the microbial source hyaluronidase. However, the bovine source products are stable for up to 1 month at 4 deg. C (500 mM acetate buffer pH 6.0) and several months at - 20 deg. C. Aliquot solutions to store frozen, avoid repeated freeze/thaw cycles. The recommended reconstitution buffer should include 0.01% BSA which acts as a stabilizer, there is no indication that the recommended sodium phosphate/sodium chloride buffer would be inappropriate for freezing.
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What is the molecular weight of Product H1136, Hyaluronidase?
1 answer-
The molecular weight for hyaluronidase from Streptomyces hyalurolyticus has been reported to be 91,000 DALTONS as determined by gel filtration.
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How should Product H1136, Hyaluronidase, be reconstituted?
1 answer-
Prior to determining the activity of this enzyme, we reconstitute it in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0.
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How is the activity of Product H1136, Hyaluronidase,determined?
1 answer-
Sigma assays the enzyme against a USP hyaluronidase reference standard as described in USP XXII-NF XVII, 644 (1990).
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What amount of product is present in the vial of Product H1136, Hyaluronidase?
1 answer-
The dry weight of the product is not determined. The vial should contain a minimum of 300 units of enzyme.
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I can't see any material in the container. Is material present in the container?
1 answer-
The product is lyophilized directly into the ampule and its appearance is a clear, thin film.
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Are any other components present in Product H1136, Hyaluronidase, besides the enzyme?
1 answer-
No protein or BSA has been added as a stabilizer. The enzyme was lyophilized from 0.5 mM Tris buffer, pH 7.0.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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