09-822
Anti-KLF5 (Krüppel-like factor 5) Antibody
serum, from rabbit
Synonym(s):
BTE-binding protein 2, Basic transcription element-binding protein 2, Colon krueppel-like factor, GC box binding protein 2, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Kruppel-like factor 5, Kruppel-like factor 5 (intestinal), Tra
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
technique(s)
ChIP: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KLF5(688)
General description
Krüppel-like factors (KLFs) are zinc finger-containing transcription factors with regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. KLF5, (also known as BTEB2 and IKLF), is essential for mouse embryo development. KLF5 activates many genes during cardiovascular remodeling, including PDGFa/b, Egr-1, PAI-1, iNOS, and VEGF receptors, making KLF a potential therapeutic target for cardiovascular disease. KLF5 also plays a role in angiogenesis and in development of several tumor types including breast, prostate, bladder, colon, esophagus, and skin. KLF5 is closely related to other of the KLF family members. However, KLF5 and KLF4 exhibit opposite effects on regulation of gene transcription, despite binding to similar, if not identical, cis-acting DNA sequences. In addition, KLF4 and 5 exert contrasting effects on cell proliferation in many instances; while KLF4 is an inhibitor of cell growth, KLF5 stimulates proliferation.
Specificity
This antibody recognizes Krüppel-like factor 5 at the N-terminus.
Immunogen
Epitope: N-terminus
Recombinant protein corresponding to mouse Krüppel-like factor 5 at the N-terminus.
Application
Anti-KLF5 (Krüppel-like factor 5) Antibody is a Rabbit Polyclonal Antibody for detection of KLF5 (Krüppel-like factor 5) also known as Krüppel-like factor 5 & has been validated in WB.
Chromatin Immunoprecipitation Analysis (ChIP):
A representative lot was used by an independent laboratory in ChIP. (Ng Huck Hui, Genome Institute of Singapore)
A representative lot was used by an independent laboratory in ChIP. (Ng Huck Hui, Genome Institute of Singapore)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Pluripotent & Early Differentiation
Transcription Factors
Pluripotent & Early Differentiation
Quality
Evaluated by Western Blot in mouse embryonic stem cell chromatin extract.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Krüppel-like factor 5 on 10 µg of mouse embryonic stem cell chromatin extract.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Krüppel-like factor 5 on 10 µg of mouse embryonic stem cell chromatin extract.
Target description
~55 kDa observed. An uncharacterized band appears at ~30 kDa
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Mouse embryonic stem cell chromatin extract
Mouse embryonic stem cell chromatin extract
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chengli Dou et al.
Journal of molecular cell biology, 15(7) (2023-08-03)
The effective proliferation and differentiation of trophoblast stem cells (TSCs) is indispensable for the development of the placenta, which is the key to maintaining normal fetal growth during pregnancy. Kruppel-like factor 5 (Klf5) is implicated in the activation of pluripotency
Zhenyu Yang et al.
Oncogene, 43(23), 1779-1795 (2024-04-23)
Transcription factors (TFs) engage in various cellular essential processes including differentiation, growth and migration. However, the master TF involved in distant metastasis of nasopharyngeal carcinoma (NPC) remains largely unclear. Here we show that KLF5 regulates actin remodeling to enhance NPC
Dong Han et al.
Science advances, 8(7), eabe4375-eabe4375 (2022-02-17)
Oct4 collaborates primarily with other transcriptional factors or coregulators to maintain pluripotency. However, how Oct4 exerts its function is still unclear. Here, we show that the Oct4 linker interface mediates competing yet balanced Oct4 protein interactions that are crucial for
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