L6522

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse

clone HP-6054, ascites fluid

• Synonym(s): Monoclonal Anti-Human Lambda Light Chains (Bound and Free)
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162516
• Conjugate: unconjugated
• Clone: HP-6054, monoclonal
• Application: ELISA (i), IHC, IP, RIA, WB
• Citations: 5

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: secondary antibodies
• clone: HP-6054, monoclonal
• contains: 15 mM sodium azide
• technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 1:1,000 using using 2 μg/ml of human IgG myeloma containing lambda light chains as the coating protein, radioimmunoassay: suitable, western blot: suitable
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Human Lambda Light Chains (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either κ or λ in which multiple structural differences are reflected in antigenic variety, mainly the N-terminal (variable) domain of the chains.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads.

Application

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse has been used:

• in immunohistochemistry
• in enzyme-linked immunosorbent assay (ELISA) assay
• in immunohistology
• in radioimmunoassay (RIA)
• in precipitation assays
• in immunoblotting
• as capture antibodies in quantitative ELISA[1]

Biochem/physiol Actions

Immunoglobulins (Ig) are produced by the B cells and play crucial role in host humoral immune response. A typical immunoglobulin consists of two heavy chains and two light chains. The two light chains are either κ or the λ chain. The B cells express one type of light chain during their life time.

The antibody is specific for human lambda (λ) light chains. This antibody is reactive with intact IgG (λ), IgA (λ), free lambda light chains and Bence-Jones lambda light chains. It is non-reactive with kappa light chains.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable