K4377

Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal

clone KP-53, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Human Kappa Light Chain (Bound and Free)
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162517
• Conjugate: unconjugated
• Technique(s): indirect ELISA: 1:10,000
• Application: ELISA (i)
• Citations: 10

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: secondary antibodies
• clone: KP-53
• contains: 15 mM sodium azide
• technique(s): indirect ELISA: 1:10,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2.

Application

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for:

• immunodetection
• in western blot analysis
• in intracellular kappa light chain staining for flow cytometric analysis

Biochem/physiol Actions

The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable