CHP2NC

Imprint^® Ultra Chromatin Immunoprecipitation Kit, Without Controls

ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

• NACRES: NA.54
• UNSPSC Code: 41116158

Description

General description

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 10⁶ cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 10⁸ cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.

Application

• Suitable for downstream applications
• Individual target characterization to genome-wide profiling techniques
• Characterization of signal transduction pathways
• Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

• Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
• Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 10⁶
• Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 10⁶ cells
• Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Only

• Sodium dodecyl sulfate solution, 10% in 18 megohm water
• Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

Kit Components Also Available Separately

• S6576

  ChIP Next Gen Seq Sepharose^™

  SDS
• I8896

  IGEPAL^® CA-630, Molecular Biology

  SDS
• S5150

  Sodium chloride solution, 5 M in H₂O, BioReagent, Molecular Biology

  SDS
• W4502

  Water, Nuclease-Free Water, for Molecular Biology

  SDS
• I5006

  IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

  SDS
• I5381

  IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder

  SDS
• A7638

  Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

  SDS

See All (12)

Safety Information

• pictograms: GHS02,GHS03,GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• target_organs: Central nervous system
• Storage Class: 5.1A - Strongly oxidizing hazardous materials
• wgk: WGK 3
• hcodes: H226,H271,H290,H302,H314,H334,H336,H410,EUH430
• pcodes: P201 - P202 - P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3