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S4030

Dextran Sulfate Solution

50% (w/v) liquid

Synonym(s):

dextran sulfate buffer, hybridization accelerant, hybridization additive, northern blot hybridization component, southern blot hybridization component

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About This Item

UNSPSC Code:
41116012
eCl@ss:
32160405
NACRES:
NA.52
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Product Name

Dextran Sulfate 50% Solution,

packaging

pkg of 100 mL

Quality Segment

manufacturer/tradename

Chemicon®

technique(s)

hybridization: suitable (nucleic acid)

shipped in

ambient

General description

Dextran Sulfate (MW >500,000) is used as an additive in probe hybridization solutions. Its presence accelerates the rate of hybridization by 10 fold. The addition of Dextran Sulfate favors the formation of probe networks, resulting in greater sensitivity during membrane-bound nucleic acid analysis. The product is supplied as a 50% (w/v) solution.

Application

Dextran Sulfate - 50% Solution

To prepare a solution containing 10% Dextran Sulfate, add 8 ml of hybridization ingredients to 2 ml of 50% Dextran Sulfate Solution.



See the "Hybrisol Nomograph for Dextran Sulfate" link located under the "Protocols" tab at catalog number S4030 product specific page on www.chemicon.com for determining the proper concentration of dextran sulfate needed for various hybridization condition guidelines.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
S4031D2532H7033
description

-

description

-

description

for Northern and Southern blotting, solution

description

for Northern and Southern blotting, solution

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

packaging

pkg of 100 mL

packaging

pkg of 200 mL

packaging

-

packaging

-

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

200

technique(s)

hybridization: suitable (nucleic acid)

technique(s)

hybridization: suitable (nucleic acid)

technique(s)

Southern blotting: suitable

technique(s)

-

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

-


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Storage Class

10 - Combustible liquids

WGK

WGK 2



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Questions

1–2 of 2 Questions  
  1. Is the product certified nuclease free?

    1 answer
    1. This product is not tested for the presence of nuclese or protease. Please see the link below to review an RNase, DNase, and Protease-free product option:
      https://www.sigmaaldrich.com/product/mm/3730op

      Helpful?

  2. Is this in the form of dextran sulfate sodium salt?

    1 answer
    1. This product is prepared from Dextran Sulfate Sodium Salt, CAS 9011-18-1. The solution is not tested for sodium content during or following preparation.

      Helpful?

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