Likely temperature needs to be optimized. It is suggested to try a range of temperatures (37, 40, 43) and to adjust stringency conditions if the background is too high.
H7033
PerfectHyb™ Plus Hybridization Buffer
for Northern and Southern blotting, solution
Synonym(s):
hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
Pricing and availability is not currently available.
General description
Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.
Application
Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.
Features and Benefits
- Fast and easy-to-use
- Up to 20x more sensitive than conventional buffers
- Doesn′t require blocking steps
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent, colorimetric , and DIG detection assays
- Compatible with radioactive and non-radioactive probes
- Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes
Other Notes
A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.
Legal Information
PerfectHyb is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form solution | form liquid (2x concentrate) | form solution | form solution |
| storage temp. room temp | storage temp. −20°C | storage temp. room temp | storage temp. room temp |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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When using H7033 with a 122-base long oligo as a probe for hybridization, should the DNA hybridization temperature or the high end of the oligonucleotide temperature be used?
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