Key Documents

Coomassie Brilliant blue G 250 (C.I. 42655)

Name: Coomassie Brilliant blue G 250 (C.I. 42655)
Brand: MM

for electrophoresis

Synonym(s):

Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain

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About This Item

Empirical Formula (Hill Notation):

C₄₇H₄₈N₃NaO₇S₂

CAS Number:

6104-58-1

Molecular Weight:

854.02

UNSPSC Code:

41105305

EC Index Number:

228-058-4

NACRES:

NC.01

MDL number:

MFCD00078482

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Properties

Quality Segment

100

potency

>5000 mg/kg LD₅₀, oral (Rat)

technique(s)

protein staining: suitable

pH

6.4 (20 °C, 10 g/L in H₂O)

solubility

40 g/L

bulk density

520 kg/m³

storage temp.

no temp limit

SMILES string

[Na+].[S](=O)(=O)([O-])c1cc(ccc1)CN(CC)c2cc(c(cc2)[C+](c5c(cc(cc5)N(CC)Cc6cc(ccc6)[S](=O)(=O)[O-])C)c3ccc(cc3)Nc4ccc(cc4)OCC)C

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1

InChI key

CMGWKYXJIYYESN-UHFFFAOYSA-M

Description

General description

Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye.[1] CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.[2] CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.[1]

Application

Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.[3] It has also been used in Bradford protein assay.[4]

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ_max. (buffer pH 7.0): 577 - 584 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for electrophoresis: passes test

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This Item	1.12553	B0149	B0770
Sigma-Aldrich 1.15444 Coomassie Brilliant blue G 250 (C.I. 42655)	Sigma-Aldrich 1.12553 Coomassie Brilliant blue R 250 (C.I. 42660)	Sigma-Aldrich B0149 Brilliant Blue R	Sigma-Aldrich B0770 Brilliant Blue G
technique(s) protein staining: suitable	technique(s) protein staining: suitable	technique(s) microbe id \| staining: suitable	technique(s) microbe id \| staining: suitable
solubility 40 g/L	solubility 20 g/L	solubility H₂O: 10 mg/mL	solubility H₂O: 1 mg/mL
Quality Level 100	Quality Level 100	Quality Level 200	Quality Level 200
storage temp. no temp limit	storage temp. 2-30°C	storage temp. room temp	storage temp. room temp
pH 6.4 (20 °C, 10 g/L in H₂O)	pH 6.2 (25 °C, 10 g/L in H₂O)	pH -	pH -
bulk density 520 kg/m³	bulk density 250 kg/m³	bulk density -	bulk density -

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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