03708152001
Roche
Streptavidin Mutein Matrix
suspension, pkg of 5 mL
About This Item
Recommended Products
form
suspension
packaging
pkg of 5 mL
manufacturer/tradename
Roche
parameter
4-25 °C optimum reaction temp.
shipped in
wet ice
General description
Settled resin of 50% suspension Streptavidin Mutein Matrix.
Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10-7 M.
The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery.
Application
- Streptavidin Mutein Matrix: Is highly suitable for purification of biotinylated proteins
- Has been tested for use with in vitro expressed biotinylated proteins, using the RTS AviTag Biotinylation Kits
- Is convenient for purification of chemically biotinylated proteins
- It was also used in single-step affinity chromatography and affinity chromatography.[1][2]
Features and Benefits
- Mild purification conditions: Low biotin dissociation constant allows elution of biotinylated proteins under gentle conditions.
- Stable: No significant loss of binding capacity upon storage. The low dissociation constant of the streptavidin mutein is obtained by genetic engineering and not by dissociation of avidin- or streptavidin tetramers.
- Economic: High binding capacity and multiple reuse allows economic handling of the matrix.
Storage and Stability
Other Notes
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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