L3397

LB Broth (Luria low salt)

Powder microbial growth medium

• Synonym(s): Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
• UNSPSC Code: 41106200
• NACRES: NA.85

Properties

• grade: for molecular biology
• Quality Level: 200
• sterility: non-sterile
• form: powder
• composition: NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• technique(s): microbiological culture: suitable
• pH: 6.8-7.2(1.55% solution)
• suitability: suitable for molecular biology; nonselective for Escherichia coli; nonselective for coliforms
• application(s): food and beverages; microbiology
• storage temp.: room temp

Description

General description

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Application

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Features and Benefits

Luria′s low salt LB powder provides:

• Standard formulation
• A budget-friendly alternative to liquid
• Easy scale-up using larger package sizes

Preparation Note

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Reconstitution

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable