Skip to Content
Merck
All Photos(1)

Key Documents

L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

Synonym(s):

Luria Broth Miller, Luria-Bertani broth, Miller

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106200
NACRES:
NA.85

grade

for molecular biology

Quality Level

400

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.2

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Application

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

Features and Benefits

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

related product

Product No.
Description
Pricing

L3147

LB Broth with agar (Miller), Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

L7658

LB Broth (Lennox), EZMix powder microbial growth medium

L3022

LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L3522

LB Broth (Miller), Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

B11001

EnPresso® B, Growth system for expressing protein in bacteria

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50
Antonio Basanta, Beatriz Gomez-Sala, et al.
Biotechnology, 76, 3314-3324 (2010)
Long-term retention of hydrophilic behavior of plasma treated polydimethylsiloxane (PDMS) surfaces stored under water and Luria-Bertani broth
Zhao LH, et al.
Sensors and actuators A, Physical, 181.0, 33-42 (2012)
Celine Abueva et al.
International journal of biological macromolecules, 182, 1713-1723 (2021-05-30)
The importance of developing more potent antimicrobials and robust infection prevention practices has been highlighted recently with the increase in reports of emerging bacterial resistance mechanisms and the development of antibiotic-resistant microbes. In this study, a quaternary ammonium chitosan derivative
Prapassorn Bussaman et al.
Journal of Zhejiang University. Science. B, 13(4), 261-266 (2012-04-03)
Xenorhabdus bacterium has been used as a biological control agent against Luciaphorus sp., a mushroom mite endemic in Thailand. To develop an effective formulation of Xenorhabdus stokiae, treatments using different parts of X. stokiae isolate PB09 culture, including whole cell
Felix Wezel et al.
International journal of molecular sciences, 22(6) (2021-04-04)
Invasive urothelial carcinomas of the bladder (UCB) characteristically show a loss of differentiation markers. The transcription factor Grainyhead-like 3 (GRHL3) plays an important role in the development and differentiation of normal urothelium. The contribution to UCB progression is still elusive.
View All Related Papers

Articles

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service