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H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max™

liquid, sterile-filtered, BioReagent, suitable for hybridoma

Synonym(s):

Histopaque 1077

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

400

product line

BioReagent

form

liquid

technique(s)

cell culture | hybridoma: suitable
single cell analysis: suitable

impurities

endotoxin, tested

density

1.077 at 25 °C (relative density)(lit.)

storage temp.

2-8°C

General description

Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Application

Histopaque®-1077 Hybri-Max™ has been used to isolate:
  • peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
  • immune cells (lymphocytes) from peripheral blood
  • PBMCs from blood

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Siddharth Sharma et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(2), 160-167 (2008-01-15)
Coulter volume is far more accurate measure of cell volume than forward angle light scatter. In this report, we have used Coulter volume to determine the mean cell volume and diameter of normal human peripheral blood cells and hematopoietic progenitor
Joshua E Messinger et al.
mBio, 10(4) (2019-07-04)
Deciphering the molecular pathogenesis of virally induced cancers is challenging due, in part, to the heterogeneity of both viral gene expression and host gene expression. Epstein-Barr virus (EBV) is a ubiquitous herpesvirus prevalent in B-cell lymphomas of immune-suppressed individuals. EBV
Carla Oseroff et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(39), 13980-13985 (2005-09-21)
We have analyzed by ex vivo ELISPOT the anti-vaccinia cytotoxic T lymphocyte responses of peripheral blood mononuclear cells from humans vaccinated with Dryvax vaccine. More than 6,000 peptides from 258 putative vaccinia ORFs predicted to bind the common molecules of
Janet E Alexander et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 73(6), 720-728 (2017-11-11)
As in human populations, advances in nutrition and veterinary care have led to an increase in the lifespan of companion animals. Detrimental physiological changes occurring later in life must be understood before interventions can be made to slow or reduce
Elliott D SoRelle et al.
eLife, 10 (2021-01-28)
Lymphoblastoid cell lines (LCLs) are generated by transforming primary B cells with Epstein-Barr virus (EBV) and are used extensively as model systems in viral oncology, immunology, and human genetics research. In this study, we characterized single-cell transcriptomic profiles of five
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Protocols

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

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