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F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

Synonym(s):

Ready-to-use density gradient media

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About This Item

CAS Number:
26873-85-8
MDL number:
MFCD00081599
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

0.2 μm filtered

Quality Level

200

type

Type 400

form

liquid

concentration

20% in H2O

technique(s)

titration: suitable

color

clear colorless

solubility

water: soluble

density

1.055-1.075 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

Related Categories

General description

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Application

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000347993
0000347993
0000347992
SLCQ8241
SLCM9158

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Junchao Xing et al.
Tissue engineering. Part C, Methods, 25(3), 137-147 (2019-02-09)
Patient-specific individual tissue-engineered bones (iTEBs) have been recognized as a promising strategy for treating large bone defects. However, current construction protocols of iTEBs vary between lots and lack standardization and quality control, hampering further research and application. This study was
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
Salmani F, et al.
Annals of Experimental Biology, 2(3), 1-7 (2014)
Juliane Schmidt et al.
PloS one, 6(3), e18184-e18184 (2011-03-30)
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have
Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
Xiaoliang Wu et al.
Shock (Augusta, Ga.), 51(6), 718-724 (2018-08-07)
The aim of this study was to investigate the correlation between plasma sphingosine-1-phosphate (S1P) and ceramide concentrations in sepsis, and the possible mechanisms for altered expression. Plasma S1P and ceramide concentrations were measured by HPLC-ESI-MS/MS. HLA-DR (human leukocyte antigen-DR) expression
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Protocols

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Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

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