67060

Methyl Green

zinc chloride salt, for microscopy (Bact., Bot., Hist.)

• Synonym(s): Ethyl Green
• Linear Formula: C₂₇H₃₅BrClN₃ · ZnCl₂
• CAS Number: 7114-03-6
• Molecular Weight: 653.24
• Colour Index Number: 42590
• Beilstein: 3914106
• EC Number: 230-415-4
• MDL number: MFCD00151094
• UNSPSC Code: 12171500
• PubChem Substance ID: 329760277
• NACRES: NA.47

Properties

• grade: for microscopy (Bact., Bot., Hist.)
• form: powder
• technique(s): titration: suitable
• pH: 0.2-1.8, yellow to blue
• mp: >300 °C (lit.)
• solubility: water: 0.01 g/10 mL, blue to very deep blue
• εmax: ≥900 at 624-634 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
• InChI: 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
• InChI key: RRKSQWOQZFIQKW-UHFFFAOYSA-J

Description

Application

Methyl green has been used in the histological staining of ovaries. It has also been used as a counterstain in various cells.

Biochem/physiol Actions

Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.

Analysis Note

Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts.

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H314,H335,H411
• Precautionary Statements: P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves