198080

Methyl Green

zinc chloride salt, certified by the Biological Stain Commission, Dye content 85 %

• Synonym(s): Ethyl Green
• Linear Formula: C₂₇H₃₅BrClN₃ · ZnCl₂
• CAS Number: 7114-03-6
• Molecular Weight: 653.24
• Colour Index Number: 42590
• Beilstein: 3914106
• EC Number: 230-415-4
• MDL number: MFCD00151094
• UNSPSC Code: 12171500
• PubChem Substance ID: 24851860
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, 85%
• technique(s): titration: suitable
• pH: 0.2-1.8, yellow to blue
• mp: >300 °C (lit.)
• solubility: H₂O: 1 mg/mL
• λmax: 423 nm (2nd); 629 nm
• ε (extinction coefficient): ≥11100 at 417-425 in H₂O at 0.005 g/L; ≥16000 at 311-319 in H₂O at 0.005 g/L; ≥60000 at 626-634 in H₂O at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
• InChI: 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
• InChI key: RRKSQWOQZFIQKW-UHFFFAOYSA-J

Description

General description

Methyl Green is a basic dicationic dye and is composed of triphenylmethane. It is suitable for the detection of DNA and RNA. It acts as a photochromophore and used to activate gelatinous films. At a very high pH, methyl green forms an alkaline solution and gets converted to a colorless carbinol base. In the presence of hydroxide anion, methyl green produces crystal violet dye and ethanol.

Suitability

Suitable for use as a DNA stain.

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H314,H335,H411
• Precautionary Statements: P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves