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Key Documents

AB5042

Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody

CHEMICON®, rabbit polyclonal

Synonym(s):

ChAT

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-Choline Acetyltransferase Antibody, serum, Chemicon®

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

guinea pig, rat, avian

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

chicken ... Chat(395314)
rat ... Chat(290567)

Specificity

Will stain cholinergic neurons in rat central and peripheral nervous systems.

Immunogen

A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. Due to sequence similarity, the antibody is expected to react with pig, rat, mouse and human ChAT.

Application

Detect Choline Acetyltransferase using this Anti-Choline Acetyltransferase Antibody validated for use in IH.
Immunohistochemistry: 1:2,000-1:4,000 in rat basal forebrain.

Western blot

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Physical form

Lyophilized. Reconstitute with 100 μL of sterile distilled water. Contains no preservative.

Storage and Stability

Maintain lyophilized material dry at -20°C or -70°C for up to 6 months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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West Nile virus-induced acute flaccid paralysis is prevented by monoclonal antibody treatment when administered after infection of spinal cord neurons.
Morrey, JD; Siddharthan, V; Wang, H; Hall, JO; Skirpstunas, RT; Olsen, AL; Nordstrom et al.
Journal of Neurovirology null
Ying Li et al.
Anatomical record (Hoboken, N.J. : 2007), 294(5), 847-857 (2011-03-19)
Amyotrophic lateral sclerosis (ALS) is a progressively fatal, incurable, neurodegenerative disorder. In this study, we investigated whether olfactory ensheathing cells (OEC) transplantation could provide protection to motor neurons and enable remyelination in mutant SOD1(G93A) transgenic rats with ALS. Seventy-two rats
Steffen Wolter et al.
Frontiers in neural circuits, 12, 65-65 (2018-10-03)
Sensory axon T-like branching (bifurcation) in neurons from dorsal root ganglia and cranial sensory ganglia depends on the molecular signaling cascade involving the secreted factor C-type natriuretic peptide, the natriuretic peptide receptor guanylyl cyclase B (GC-B; also known as Npr2)
Ritchie E Brown et al.
The European journal of neuroscience, 27(2), 352-363 (2008-01-25)
Recent experiments suggest that brainstem GABAergic neurons may control rapid-eye-movement (REM) sleep. However, understanding their pharmacology/physiology has been hindered by difficulty in identification. Here we report that mice expressing green fluorescent protein (GFP) under the control of the GAD67 promoter
Erin E Maher et al.
The Journal of comparative neurology, 529(10), 2558-2575 (2021-01-19)
Ventroposterior medialis parvocellularis (VPMP) nucleus of the primate thalamus receives direct input from the nucleus of the solitary tract, whereas the homologous thalamic structure in the rodent does not. To reveal whether the synaptic circuitries in these nuclei lend evidence

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