201677

Janus Green B

certified by the Biological Stain Commission, Dye content 65 %

• Synonym(s): 3-Diethylamino-7-(4-dimethylaminophenylazo)-5-phenylphenazinium chloride, Diazin Green S, Union Green B
• Empirical Formula (Hill Notation): C₃₀H₃₁ClN₆
• CAS Number: 2869-83-2
• Molecular Weight: 511.06
• Colour Index Number: 11050
• EC Number: 220-695-6
• MDL number: MFCD00011758
• UNSPSC Code: 12171500
• PubChem Substance ID: 24852018
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, 65%
• technique(s): microbe id | staining: suitable
• color: black; very dark brown; very dark green
• λmax: 395 nm; 660 nm (2nd)
• ε (extinction coefficient): ≥28000 at 651-677 nm in ethanol: water (1:1) at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: [Cl-].CCN(CC)c1ccc2nc3ccc(cc3[n+](-c4ccccc4)c2c1)\N=N\c5ccc(cc5)N(C)C
• InChI: 1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1
• InChI key: XXACTDWGHQXLGW-UHFFFAOYSA-M

Description

Application

Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.

Biochem/physiol Actions

Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves