A4043

Azure B

Dye content, ≥89%, certified by the Biological Stain Commission, powder

• Synonym(s): N,N,N′-Trimethylthionin, Azure I, Methyleneazure
• Empirical Formula (Hill Notation): C₁₅H₁₆ClN₃S
• CAS Number: 531-55-5
• Molecular Weight: 305.83
• Colour Index Number: 52010
• Beilstein: 3922630
• EC Number: 208-511-2
• MDL number: MFCD00011935
• UNSPSC Code: 12171500
• PubChem Substance ID: 24890740
• NACRES: NA.47

Properties

• product name: Azure B, certified by the Biological Stain Commission
• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, ≥89%
• technique(s): microbe id | staining: suitable
• color: dark green
• mp: 205-210 °C (dec.) (lit.)
• solubility: H₂O: 1 mg/mL
• fluorescence: λ_ex 648 in ethanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]
• InChI: 1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H
• InChI key: DNDJEIWCTMMZBX-UHFFFAOYSA-N

Description

Application

Azure B has been used for the staining of chromosomes.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.

Suitability

Certified for use in Lillie′s modified Nocht′s method for paraffin sections and the NCCLS method for blood smears.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Precautionary Statements: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves