An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K. These columns should be used with non-aqueous mobile phases only.
SUPELCOSIL LC-NH2-NP is an amino phase dedicated to normal phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal phase chromatography.
Features and Benefits
• show stable retention in normal-phase separations • are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica • provide excellent separations of fat-soluble vitamins
SUPELCOSIL LC-NH2-NP columns: • show stable retention in normal phase separations • are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica • provide excellent separations of fat-soluble vitamins
Recommended products
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 799(1), 165-172 (2003-12-09)
The aim of this study was to develop a specific and sensitive high-performance liquid chromatographic (HPLC) assay for the determination of levofloxacin in human plasma, bronchoalveolar lavage and bone tissues. The sample extraction was based on a fully automated liquid-solid
A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed and validated for the determination of DRF-1042, a novel orally active camptothecin (CPT) analog, in human plasma. The sample preparation was a simple deproteinization with acidified methanol yielding
Journal of chromatography. A, 692(1-2), 103-119 (1995-02-10)
A comprehensive approach to the analysis for many drugs in postmortem blood and biological fluids using high-performance liquid chromatography and diode array detection has been developed. To reduce the likelihood of co-eluting interference components of postmortem blood or other drugs
International journal of pharmaceutical compounding, 4(3), 229-231 (2000-05-01)
A simple and selective high-performance liquid chromatography (HPLC) method using ultraviolet detection was developed for simultaneous determination of fusidic acid and betamethasone dipropionate in a cream formulation. A Supelcosil LC18 column was used for chromatographic separation. The mobile phase consisted
Journal of chromatographic science, 46(2), 169-173 (2008-03-28)
A sensitive, simple, and reliable high-performance liquid chromatographic method with electrochemical detection is developed for the measurement of four natural products, the serotonin-related indols from human platelet-rich plasma (PRP) using N-methylserotonin as internal standard. Separation of serotonin (5HT), 5-hydroxytryptophan (5HTP)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.