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Trypsin Agarose

Name: Trypsin Agarose
Brand: SIGMA

buffered aqueous suspension, from bovine pancreas trypsin

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About This Item

UNSPSC Code:

12352204

eCl@ss:

42020142

NACRES:

NA.54

EC Number:

3.4.21.4 (BRENDA, IUBMB)

MDL number:

MFCD00082094

Biological source:

bovine pancreas (trypsin)

Concentration:

≥15 units/mL (packed gel)

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Properties

biological source

bovine pancreas (trypsin)

Quality Segment

200

form

buffered aqueous suspension

concentration

≥15 units/mL (packed gel)

extent of labeling

≥15 units per mL packed gel

matrix

cross-linked beaded agarose

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides.

A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. [1] Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography. [2]

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2

Other Notes

Insolubilized

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

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1 of 1

This Item	T4019	C1261	T8802
Sigma-Aldrich T1763 Trypsin Agarose	Sigma-Aldrich T4019 Trypsin−Agarose	Sigma-Aldrich C1261 Carboxypeptidase A−Agarose	Sigma-Aldrich T8802 Trypsin from bovine pancreas
biological source bovine pancreas (trypsin)	biological source bovine pancreas (trypsin)	biological source enzyme from bovine pancreas	biological source bovine pancreas
form buffered aqueous suspension	form buffered aqueous suspension	form ammonium sulfate suspension	form essentially salt-free, lyophilized powder
concentration ≥15 units/mL (packed gel)	concentration ≥15 units/mL (packed gel)	concentration -	concentration -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
shipped in wet ice	shipped in -	shipped in -	shipped in -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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pictograms

GHS07

signalword

Warning

hcodes

H315,H317,H319,H335

pcodes

P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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