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Carboxypeptidase A−Agarose

Name: Carboxypeptidase A−Agarose
Brand: SIGMA

ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

Synonym(s):

Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.17.1 (BRENDA, IUBMB)

MDL number:

MFCD00130718

Specific activity:

≥6 units/mL packed gel, 25 °C

Biological source:

enzyme from bovine pancreas

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Properties

biological source

enzyme from bovine pancreas

form

ammonium sulfate suspension

specific activity

≥6 units/mL packed gel, 25 °C

mol wt

~35,250

matrix

beaded agarose

storage temp.

2-8°C

Description

General description

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.[1]

Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.

Physical form

Suspension in 2.0 M (NH₄)₂SO₄, pH 7

Other Notes

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

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This Item	C9268	R4001	T1763
Sigma-Aldrich C1261 Carboxypeptidase A−Agarose	Sigma-Aldrich C9268 Carboxypeptidase A from bovine pancreas	Sigma-Aldrich R4001 Ribonuclease A-agarose	Sigma-Aldrich T1763 Trypsin Agarose
biological source enzyme from bovine pancreas	biological source -	biological source protein from bovine pancreas	biological source bovine pancreas (trypsin)
specific activity ≥6 units/mL packed gel, 25 °C	specific activity ≥50 units/mg protein	specific activity 400-1,000 units/g agarose (One ml gel will yield 12-30 units)	specific activity -
form ammonium sulfate suspension	form ready-to-use solution	form ammonium sulfate suspension	form buffered aqueous suspension
mol wt ~35,250	mol wt ~35 kDa	mol wt ~13,700	mol wt -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 300	Quality Level -	Quality Level 200

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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