SAB3701431

Monoclonal Anti-Esrp-1/2 antibody produced in mouse

clone 23A7.C9, purified immunoglobulin

• Synonym(s): Anti-Epithelial splicing regulatory protein 1, Anti-RNA-binding motif protein 35A, Anti-RNA-binding protein 35A, Anti-RNA-binding protein 35B, Anti-Rbm35a
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 23A7.C9, monoclonal
• form: buffered aqueous solution
• species reactivity: mouse
• technique(s): ELISA: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: Q8K0G8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Esrp2(77411)

Description

General description

Epithelial splicing regulatory protein 1 (ESRP1) is a member of RBM family of RNA-binding proteins. It is also known as RBM35A. ESRP1 get highly expressed in both mouse and human epithelial stem cells and weakly expressed in normal pancreatic ductal cells.

Specificity

This antibody was purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins.  A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee .  The binding epitope of this monoclonal antibody has not been mapped

Immunogen

Full length recombinant mouse Esrp-1 fusion protein.

Biochem/physiol Actions

Epithelial splicing regulatory protein 1 (ESRP1) is essential for the expression of epithelial FGFR2-IIIb (fibroblast growth factor receptor 2). Suppression of ESRP1 can enhance induced pluripotent stem (iPS) cell colony generation. It can also repress cancer cell motility by different mechanisms. ESRP1 modulates splicing of Ras-related C3 botulinum toxin substrate 1 (Rac1) isoforms and participates in the maintenance of epithelial cell-specific isoforms. The protein controls epithelial splicing regulatory network and pluripotency.

Features and Benefits

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Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable